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Erythrocyte Amyloid Beta Peptide Isoform Distributions in Alzheimer and Mild Cognitive Impairment

Author(s):

Petter Järemo*, Alenka Jejcic, Vesna Jelic, Tasmin Shahnaz, Magnus Oweling, Bengt Winblad and Homira Behbahani   Pages 1 - 5 ( 5 )

Abstract:


Introduction: We recently showed that amyloid beta (Aβ) 40 accumulates in erythrocytes and possibly causes cell damage as evidenced from an increased number of assumed injured low-density (kg/L) erythrocytes. Furthermore, we have suggested a separation technique to isolate and concentrate such damaged red blood cells for subsequent analysis.

Objectives: We isolated high- and low-density erythrocytes and investigated the accumulation patterns of the Aβ peptides (Aβ 40 , Aβ 42, and Aβ 43 ) in Alzheimer (AD), mild cognitive impairment (MCI), and subjective cognitive impairment (SCI).

Methods: Whole blood was fractionated through a density gradient, resulting in two concentrated high- and presumed injured low-density erythrocyte fractions. After cell lysis, intracellular Aβ 40 , Aβ 42, and Aβ 43 were quantified by ELISA.

Results: In both high- and low-density erythrocytes, the Aβ 40 displayed the lowest concentration in MCI, while it was equal and higher in AD and SCI. Aβ 40 was detected at a 10- fold higher level than Aβ 42, and in injured low-density erythrocytes, the lowest quantity of Aβ 42 was found in AD and MCI. Aβ 40 exhibited a 100-fold greater amount than Aβ 43, and lighter erythrocytes of MCI subjects displayed less intracellular Aβ 43 than SCI.

Conclusion: Red blood cell accumulation patterns of Aβ 40, Aβ 42, and Aβ 43 differ significantly between AD, MCI, and SCI. The data must be verified through larger clinical trials. It is, however, tenable that Aβ peptide distributions in erythrocyte subpopulations have potentials to be used for diagnostic purposes.

Keywords:

Alzheimer, amyloid-β40, amyloid-β42, amyloid-β43, erythrocytes, MCI, SCI

Affiliation:

Department of Internal Medicine, Vrinnevi Hospital, Norrköping, Department of Internal Medicine, Vrinnevi Hospital, Norrköping, Memory Clinic, Theme Aging, Karolinska University Hospital Huddinge, Department of Internal Medicine, Vrinnevi Hospital, Norrköping, Department of Internal Medicine, Vrinnevi Hospital, Norrköping, Department of Neurobiology, Care Sciences, and Society, Center for Alzheimer Research, Division of Neurogeriatrics, Karolinska Institutet, Solna, Department of Neurobiology, Care Sciences, and Society, Center for Alzheimer Research, Division of Neurogeriatrics, Karolinska Institutet, Solna



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